Coding

Part:BBa_K1076003

Designed by: Carlos Gustavo Nunes   Group: iGEM13_Manaus_Amazonas-Brazil   (2013-09-13)

FadD, Long-chain-fatty-acid-CoA ligase

FadD ligates CoA in fatty acid chain preparing it to get into the Beta-Oxidation metabolic pathway.We are expressing this coding sequence (ORF) in order to increase B-oxidation and therefore ATP, H+ and electrons production in Shewanella putrefaciens.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1477
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1631
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1210
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 163


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Categories
Parameters
None